Author + information
- J.B. Kim,
- J.S. Woo,
- W.S. Kim,
- W. Kim,
- K.S. Kim and
- J.M. Lee
Dietary n-3 polyunsaturated fatty acids (PUFAs) have been known to reduce risk of coronary heart disease and sudden cardiac death (SCD). But limited data were available for acute ischemic/reperfusion model with regular dose n-3 PUFAs supply for short term period.
Fourteen adult male Sprague-Dawley rats were used. Normal saline and 100mg of omega-3(Omacor® 100mg daily; consist of EPA 46mg and DHA 38mg) were injected into intraperitoneum daily to each group for 10 days (control group (n = 7), Omega-3(n = 7)). LAD(left anterior descending) artery was ligated and reperfused for 5 minutes. In vivo ventricular arrhythmia induction was done with electrical stimulator. Optical mapping was performed. ECG was recorded during all procedure and HRV(heart rate variability) ratio was acquired.
There were no significant difference between control and omega-3 group in Ventricular tachyarrhythmia(VT/VF) occurrence in LAD ligation and reperfusion state.(During LAD ligation : 1.1±1.2 times in control group vs 0.5±0.6 times in omega-3 group, p=0.299; During reperfusion state : 4.7±3.6 times in control group vs 2.7±2.0 in omega-3 group p=0.351). In vivo ventricular arrhythmia induction test, VT/VF induction rate showed no difference between two groups(6.6±4.2 times vs 3.1±2.1 times for 10 electrical stimulation, p=0.091). There were also no significant difference in HRV ratio(1.28±0.74 in control group vs 1.12±0.62 in omega-3 group, p=0.837). In optical mapping, there were no significant difference of action potential duration at 70%(APD70) between control and omega-3 group at normal(37.1±32.2ms vs 33.0±34.3ms), borderline area(59.7±51.8ms vs 80.8±48.7ms) and infarction area(89.4±47.4ms vs 122.8±24.0ms) respectively(p=0.876 , p=0.343 and p=0.202).
Omega-3 was not effective in acute ischemic/reperfusion model with intraperitoneal regular dose of omega-3 for short term period. There was no significant difference in VT/VF occurrence, VT/VF induction ratio, HRV ration and APD70 between two groups.